Recombinant:Article Title: HER-2 binding antibodies
Article Snippet: .. Materials: Plates: 384 well NUNC Maxisorp plates; NUNC; Cat. No. 464718 Coating Proteins: human Her1 (Recombinant Human EGFR/ErbB1 Fc Chimera; R&D Systems; Cat. No. 344-ER) human Her2 (Recombinant Human ErbB2/HER2 Fc Chimera; R&D Systems; Cat. No. 1129-ER) human Her3 (Recombinant Human ErbB3/Her3 Fc Chimera; R&D Systems; Cat. No. 348-RB) human Her4 (Recombinant Human ErbB4/HER4 Fc Chimera; R&D Systems; Cat. No. 1131-ER) cyno Her2 (Cynomolgus HER2/ErbB2 Protein; Sino Biological; Cat. No. 90295-C08H) rat Her2 (Rat HER2/ErbB2 Protein; Sino Biological; Cat. No. 80079-RCCH) mouse Her2 (Mouse Her2/ErbB2 Protein; Acro Biosystems; Cat. No. ER2-M5220) Primary Abs: Trastuzumab Pertuzumab MABD B100 MAB-16-0160 MAB-16-0161 MAB-16-0163 MAB-16-0165 Detection Ab: anti-human Fab2 POD-Antibody; AbD Serotec; STAR126P PBS: Buffers in a Box, Premixed PBS Buffer, 10×; Roche Applied Sciences; Cat. No. 11666789001 BSA: Bovine Serum Albumin Fraction V from bovine serum; Roche Applied Sciences; Cat. No. 10735086001 Tween 20: Tween 20; Sigma-Aldrich; Cat. No. P1379 TMB: TMB Solution; Invitrogen; Cat. No. SB02 HCl: 1M Titripur Hydrochloric Acid; Merck; Cat. No. 1090571000 ELISA Buffer: PBS, 0.5% BSA, 0.05% Tween Wash Buffer: PBS, 0.1% Tween Block Buffer: PBS, 2% BSA, 0.05% Tween Samples: Dilution in ELISA buffer is project dependent (for high concentrated IgGs 1:2 dilution is recommended) Procedure: 1. ..
Enzyme-linked Immunosorbent Assay:Article Title: HER-2 binding antibodies
Article Snippet: .. Materials: Plates: 384 well NUNC Maxisorp plates; NUNC; Cat. No. 464718 Coating Proteins: human Her1 (Recombinant Human EGFR/ErbB1 Fc Chimera; R&D Systems; Cat. No. 344-ER) human Her2 (Recombinant Human ErbB2/HER2 Fc Chimera; R&D Systems; Cat. No. 1129-ER) human Her3 (Recombinant Human ErbB3/Her3 Fc Chimera; R&D Systems; Cat. No. 348-RB) human Her4 (Recombinant Human ErbB4/HER4 Fc Chimera; R&D Systems; Cat. No. 1131-ER) cyno Her2 (Cynomolgus HER2/ErbB2 Protein; Sino Biological; Cat. No. 90295-C08H) rat Her2 (Rat HER2/ErbB2 Protein; Sino Biological; Cat. No. 80079-RCCH) mouse Her2 (Mouse Her2/ErbB2 Protein; Acro Biosystems; Cat. No. ER2-M5220) Primary Abs: Trastuzumab Pertuzumab MABD B100 MAB-16-0160 MAB-16-0161 MAB-16-0163 MAB-16-0165 Detection Ab: anti-human Fab2 POD-Antibody; AbD Serotec; STAR126P PBS: Buffers in a Box, Premixed PBS Buffer, 10×; Roche Applied Sciences; Cat. No. 11666789001 BSA: Bovine Serum Albumin Fraction V from bovine serum; Roche Applied Sciences; Cat. No. 10735086001 Tween 20: Tween 20; Sigma-Aldrich; Cat. No. P1379 TMB: TMB Solution; Invitrogen; Cat. No. SB02 HCl: 1M Titripur Hydrochloric Acid; Merck; Cat. No. 1090571000 ELISA Buffer: PBS, 0.5% BSA, 0.05% Tween Wash Buffer: PBS, 0.1% Tween Block Buffer: PBS, 2% BSA, 0.05% Tween Samples: Dilution in ELISA buffer is project dependent (for high concentrated IgGs 1:2 dilution is recommended) Procedure: 1. ..
Blocking Assay:Article Title: HER-2 binding antibodies
Article Snippet: .. Materials: Plates: 384 well NUNC Maxisorp plates; NUNC; Cat. No. 464718 Coating Proteins: human Her1 (Recombinant Human EGFR/ErbB1 Fc Chimera; R&D Systems; Cat. No. 344-ER) human Her2 (Recombinant Human ErbB2/HER2 Fc Chimera; R&D Systems; Cat. No. 1129-ER) human Her3 (Recombinant Human ErbB3/Her3 Fc Chimera; R&D Systems; Cat. No. 348-RB) human Her4 (Recombinant Human ErbB4/HER4 Fc Chimera; R&D Systems; Cat. No. 1131-ER) cyno Her2 (Cynomolgus HER2/ErbB2 Protein; Sino Biological; Cat. No. 90295-C08H) rat Her2 (Rat HER2/ErbB2 Protein; Sino Biological; Cat. No. 80079-RCCH) mouse Her2 (Mouse Her2/ErbB2 Protein; Acro Biosystems; Cat. No. ER2-M5220) Primary Abs: Trastuzumab Pertuzumab MABD B100 MAB-16-0160 MAB-16-0161 MAB-16-0163 MAB-16-0165 Detection Ab: anti-human Fab2 POD-Antibody; AbD Serotec; STAR126P PBS: Buffers in a Box, Premixed PBS Buffer, 10×; Roche Applied Sciences; Cat. No. 11666789001 BSA: Bovine Serum Albumin Fraction V from bovine serum; Roche Applied Sciences; Cat. No. 10735086001 Tween 20: Tween 20; Sigma-Aldrich; Cat. No. P1379 TMB: TMB Solution; Invitrogen; Cat. No. SB02 HCl: 1M Titripur Hydrochloric Acid; Merck; Cat. No. 1090571000 ELISA Buffer: PBS, 0.5% BSA, 0.05% Tween Wash Buffer: PBS, 0.1% Tween Block Buffer: PBS, 2% BSA, 0.05% Tween Samples: Dilution in ELISA buffer is project dependent (for high concentrated IgGs 1:2 dilution is recommended) Procedure: 1. ..
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